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OBJECTIVE：To study the effects of chitosan graphene oxide car rier（CS-GO）loaded with oridonin （CS-GO- oridonin）on the proliferation and apoptosis of human lung cancer A 549 cells. METHODS ：Taking A 549 cells as objects ，the survival rate of cells were detected by CCK- 8 method after treated with different concentrations of CS-GO （3，6，12，24，48 μg/mL）and CS-GO-oridonin loaded with same mass of oridonin （3，6，12，24，48 μg/mL，by the weight of oridonin ，the same below）. IC 50 of CS-GO-oridonin was calculated. The cell morphology were observed by microscope after treated with CS-GO and CS-GO-oridonin（both 32 μg/mL）for 2，4，10，24 h. The uptake of CS-GO ，oridonin，CS-GO-orionin（all 32 μg/mL）by cells was observed with fluorescence labeling method. The apoptosis of cells and the content of ROS were observed by flow cytometry after treated with different concentrations of CS-GO （16，32，64 μg/mL）and CS-GO-oridonin （16，32，64 μg/mL）. The expression of anti-apoptosis related proteins （Mcl-1，Bax and Bak ）were detected by Western blot. RESULTS ：After treated with different concentrations of CS-GO ，the survival rate of cells was still above 90％ ；after treated with different concentrations of CS-GO-oridonin，the survival rate of cells showed a downward trend ，and was significantly lower than that of CS-GO group （P＜ 0.01）；IC50 of CS-GO-oridonin was 32.61 μg/mL. After CS-GO treatment，the cell morphology did not change significantly ；after CS-GO-oridonin treatment ，the cells shrunk and fell off in clusters ，and the suspended matter increased ；the fluorescence of oridonin and CS-GO-orionin taken up by cells was enhanced than CS-GO. Compared with blank group ，there was no significant change in the apoptosis rate ，the content of ROS and the expression of apoptosis-related protein in 16，32，64 μg/mL CS-GO groups（P＞0.05）；apoptosis rate ，the content of ROS ，the protein expression of Bax and Bak in 16，32，64 μg/mL CS- GO-oridonin groups were increased significantly ，while the protein expression of Mcl- 1 were decreased significantly. Above indexes were significantly high er or lower than the same concentration CS-GO group （P＜0.05）. CONCL USIONS：CS-GO dose not affect the proliferation and apoptosis of A 549 cells；CS-GO-oridonin has obvious inhibition and apoptosis promoting effect on cells ，which may be related to increasing ROS production and regulating the expression of apoptosis related proteins.
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Objective To establish a medical quality evaluation system for gynecology wards in specialized gynecology hospitals by means of DRGs. Methods DRGs method was used to adjust risks of the information of gynecology inpatients discharged in 2016. On such basis, the medical quality evaluation system was built on the three dimensions of medical ability, medical efficiency and medical safety, while a general scoring was obtained by multiplying the scoring of the dimension with its respective weighting coefficients. Results The general scoring of the 10 wards ranged between 0. 89-1. 08, averaging 0. 99. That of Ward B ranked the highest, and Ward I the lowest of the ten. The medical ability scoring ranged between 0. 87-1. 12, averaging 1. 00;That of Ward B ranked the highest,and Ward I the lowest of the ten. Medical efficiency scoring ranged between 0. 93 -1. 23, averaging 1. 00;That of Ward J ranked the highest,and Ward E the lowest of the ten. Safety scoring ranged between 0. 89-0. 99, averaging 0. 94; That of Ward F ranked the highest, and Ward A the lowest of the ten. Conclusions The medical quality evaluation system based on DRGs can effectively evaluate wards of a gynecology department, supporting their fine management.
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Objective To establish a medical quality evaluation system for gynecology wards in specialized gynecology hospitals by means of DRGs. Methods DRGs method was used to adjust risks of the information of gynecology inpatients discharged in 2016. On such basis, the medical quality evaluation system was built on the three dimensions of medical ability, medical efficiency and medical safety, while a general scoring was obtained by multiplying the scoring of the dimension with its respective weighting coefficients. Results The general scoring of the 10 wards ranged between 0. 89-1. 08, averaging 0. 99. That of Ward B ranked the highest, and Ward I the lowest of the ten. The medical ability scoring ranged between 0. 87-1. 12, averaging 1. 00;That of Ward B ranked the highest,and Ward I the lowest of the ten. Medical efficiency scoring ranged between 0. 93 -1. 23, averaging 1. 00;That of Ward J ranked the highest,and Ward E the lowest of the ten. Safety scoring ranged between 0. 89-0. 99, averaging 0. 94; That of Ward F ranked the highest, and Ward A the lowest of the ten. Conclusions The medical quality evaluation system based on DRGs can effectively evaluate wards of a gynecology department, supporting their fine management.
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Objective:To investigate the value of serum CD105, CA125 and CA724 levels in the treatment of cervical cancer with paclitaxel and cisplatin.Methods: A total of 42 patients with cervical cancer were randomly divided into observation group (n=21) and control group (n=21). The observation group was given intravenous infusion of paclitaxel 135 mg/m2, and intravenous infusion of cisplatin 60 mg/m2 after 3 hours. The control group was given only intravenous infusion of cisplatin, during the hydration and antiemetic drugs. They need 2 courses of chemotherapy, with each chemotherapy interval of 21 days. The levels of serum CD105, CA125 and CA724 before and after treatment were compared and analyzed.Results: The levels of CD105, CA125 and CA724 in the observation group were lower than those in the control group (t=19.119,t=22.493,t=15.46;P<0.05). There was a negative correlation between CD105, CA125 and CA724 levels and clinical efficacy (rs=-0.427,rs=-0.335,rs=-0.408;P<0.05).Conclusion: Paclitaxel combine with cisplatin in the treatment of cervical cancer has better clinical curative effect, and the mechanism is related to lower CD105, CA125 and CA724 levels.
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Objective:To study the effect of sufentanil combined with dezocine on the PCIA after abdominal operation. Methods:Totally 110 patients undergoing abdominal operation were randomly divided into the experimental group and the control group with 55 cases in each. The two groups were both treated with electronic analgesia pump for the PCIA after the operation. The control group was given dezocine 0. 3 mg·kg-1 ,and the experimental group was given sufentanil injections 0. 1 g·kg-1 plus dezocine 0. 3 mg·kg-1 . The VAS score,adverse drug reactions,Ramesay scores,press number of patient controlled analgesia,exhaust time,recovery time of bowel sounds and bowel audio rate index in the two groups were compared to evaluate the effect of PCIA. Results:All the observed in-dices in the experimental group were significantly better than those in the control group(P<0. 01). The incidence of adverse reactions in the experimental group was significantly higher than that in the control group(P<0. 01). Conclusion:Sufentanil combined with dezocine for the PCIA after abdominal operation shows significantly analgesic and sedative effects,and can notably improve the clinical symptoms,which is worthy of clinical application.
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OBJECTIVE:To study the role of clinical pharmacists in the utilization of antibacterial drugs in gynecologic inpatients. METHODS: Gynecologic inpatients in our hospital from Jun. to Dec. in 2008 were randomly divided into clinical pharmacists participation group(pharmacists group, n=158) and doctors’ experience group (experience group, n=158) according to admission order. The utilization of antibiotics during hospitalization was analyzed statistically. RESULTS: Two groups were all treated with antibacterial drugs. The utilization rate of single drug in pharmacist group was significantly higher than in experience group while the utilization rates of two-drug and three-drug combination in pharmacist group were strikingly lower than in experience group (P
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OBJECTIVE:To analyze the chemical components extracted with supercritical CO2(SFE-CO2)from Eucalyptus tereticornis.METHODS: Volatile oil was extracted from E.tereticornis with SFE-CO2.The chemical component analyzed by GC-MS and its proportion was determined by normalization method.RESULTS: 28 compounds which account for 88.13% of the total peak area were separated and identified.The main components of volatile oil were eucalyptol (33.99%),borneol(8.88%),?-pinene (5.39%),caryophyllene (4.51%),(+)-4-carene (4.19%).CONCLUSION:This study can be served as a scientific basis for the further exploitation and utilization of E.tereticornis.
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Objective:To explore the role of CD86 costimulation in inducing Th2 bias at maternal-fetal interface and the relationship to outcomes of gestation.Methods:Pregnant DBA/2J mated CBA/J mice with a high embryo resorption rate from 20% to 30% and BALB/C mated CBA/J mice with low embryo resorption rates were studied,with rat anti-murine CD86 mAb administered intraperitoneally at the dosage of 100 ?g,at the time of implantation(day 4) and on the following days(6,8,10) of gestation.The competitive semiquantity RT-PCR and ELISA was applied to analysis of the transcription and expression of Th type-1/Th type-2 cytokines at the maternal-fetal interface at day 9 or day 14 of gestation respectively.The embryo resorption rate was counted at day 14 of gestation.Results:In the model of normal pregnancy,blockade of CD86 costimulation had no significant effects on the original Th2 bias at the maternal-fetal interface,and the outcomes of gestation had not changed significantly.While in the model of abortion-prone,blockade of CD86 costimulation successfully induced a Th2 bias at maternal-fetal interface.Therefore,the embryo resorbing rates decreased significantly.Conclusion:Blocking the CD86 costimulation at the early stage of the abortion-prone pregnancy could recover the physiological balance of Th1/Th2 at maternal-fetal interface and induce the maternal-fetal immune tolerance.